Real Time PCR Laboratory

Objective

Quantification of RNA transcript level at a certain time/ condition in a cell. Primers based specific pathogens at very low threshold limit. Evaluation of the primers for specificity to the target organism, A conventional diagnostic protocol.

Equipment & Details

ABI PRISM 7000 Sequence Detection Systems
ABI PRISM 7000 Sequence Detection Systems, Applied Biosystem, USA.

Sample Details

    Samples will not be analyzed unless complete information is provided.

    Must bring your DNA samples, primers, buffer, enzymes and PCR water to set up the reaction.

    The user must bring samples to CRF on the date and time of analysis.

    Sequencing data will be provided in CD or as e-mail attachment.

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Utility and Working Principle

Real-time PCR is a thermal cycler with the capacity to fluorescence each sample with a beam of light of at least one specified wavelength and detect the fluorescence emitted by the excited fluorophore.