Fluorescence Activated Cell Sorter Lab (FACS)
Objective
To isolate and sort different cell types from organs and tissues.
To provide a fast and quantitative measurement of intra- and extracellular properties, including genotype variation of a particular type of cell.
To get quick and accurate immunological information of patients. immunophenotyping analysis of lymphomas, leukemias, and congenital immunodeficiencies; monitoring CD4+ T-lymphocyte counts.
DNA content analysis for prognosis of malignancies and screening of hematologic disorders.
Equipment & Details
FACS Aria IIIFACS Caliber provided by M/S Becton Dickinson and Company Singapore Branch, 30 Tuas Avenue, Singapore-639461.
FACS Aria III provided by M/S Becton Dickinson Holding Pte Ltd (BDHPL) Singapore Branch, 30 Tuas Avenue, Singapore-639461.
High performance analyzer cum cell sorter, Fiber optics light path-Both for lasers and Emission, 6 lasers, 2 physical (Forward Scatter and side scatter) and 9 fluorescence detectors.
Sample Details
Samples will not be analyzed unless complete information is provided.
Samples should be stained by the user.
Pathogenic sample or Human blood or tissue must be fixed otherwise will not be allowed to analysis.
The user must bring samples to CRF on the date and time of analysis.
One Hard copy of the result will be provided.
Samples should be stained by the user.
Pathogenic sample or Human blood or tissue must be fixed otherwise will not be allowed to analysis.
The user must bring samples to CRF on the date and time of analysis.
One Hard copy of the result will be provided.
Assigned Technician:
Dr. Samiran Sona Gauri
Technical Superintendentsamiran@iitkgp.ac.in
82499
Fluorescence Activated Cell Sorter Lab (FACS)
Location: NB / FF / 4 & 5, CRF
Facilitator: Prof. Sudip Kumar Ghosh
Location: NB / FF / 4 & 5, CRF
Facilitator: Prof. Sudip Kumar Ghosh
Prof. Sudip Kumar Ghosh
Biotechnology, IIT Kharagpursudip@hijli.iitkgp.ac.in
+91-3222-283768
Utility and Working Principle
It works depending upon the light scattering features of the cells, which may be derived from dyes or monoclonal antibodies targeting either extracellular molecules located on the surface or intracellular molecules inside the cell.