High End Super-resolution microscopy

ZEISS Lattice SIM 5 :

The ZEISS Lattice SIM 5 is designed to provide advanced super-resolution fluorescence imaging for visualization of biological structures (both live and fixed samples) beyond the diffraction limit of conventional light microscopy.

The system is designed to achieve super-resolution imaging in the range of 60–120 nm, delivering enhanced spatial resolution for detailed visualization of cellular structures. It enables high-speed live-cell imaging while maintaining minimal phototoxicity, ensuring cell viability during prolonged experiments. The platform provides high-contrast optical sectioning along with advanced 3D imaging capabilities, allowing precise visualization of complex biological samples. It supports long-term imaging of dynamic biological processes, making it suitable for time-lapse and functional studies. Additionally, the system facilitates multidisciplinary research across life sciences and biomedical applications by integrating Widefield, Optical Sectioning, and Structured Illumination Microscopy (SIM) imaging modes within a single unified platform.

The system enables researchers to study cellular architecture and molecular dynamics under physiologically relevant conditions.

Leica STED STELLARIS :

The system enables super-resolution imaging with an approximate resolution of 40–80 nm using Stimulated Emission Depletion (STED) technology, allowing detailed visualization of subcellular and molecular architecture at the nanoscale level. It supports multiplex fluorescence imaging, making it suitable for studying complex biological systems with multiple labelled targets simultaneously. The platform provides quantitative imaging capabilities for precise molecular localization and interaction analysis, enhancing the accuracy of biological investigations. Designed to facilitate advanced research in cell biology, neuroscience, microbiology, and biomedical sciences, the system integrates both confocal and STED imaging modes within a single platform, offering flexibility for a wide range of imaging applications.

EQUIPMENT 1 : 

Instrument Name-ZEISS Lattice SIM 5

Manufacturer-ZEISS 

EQUIPMENT 2 : 

Instrument Name-Leica STED STELLARIS Super-Resolution Confocal Microscope

Manufacturer-Leica Microsystems

ZEISS Lattice SIM 5 :

In this technique, a structured lattice illumination pattern is projected onto the sample to enhance spatial information capture. Multiple images are acquired at different illumination phases and orientations, allowing high-frequency spatial details encoded within the illumination pattern to become detectable beyond the conventional diffraction limit. The collected raw data are then processed using advanced computational reconstruction based on the SIM² algorithm, which extracts and combines the encoded information. As a result, a super- resolution image is generated with improved contrast and approximately doubled spatial resolution compared to standard widefield microscopy.

Leica STED STELLARIS :
Stimulated Emission Depletion (STED) microscopy enhances spatial resolution by selectively depleting fluorescence emission surrounding the focal excitation spot. In this technique, a focused excitation laser first excites fluorophores within the sample, followed immediately by a donut-shaped STED depletion laser. The depletion beam forces fluorophores located at the periphery back to the ground state through stimulated emission, effectively restricting fluorescence emission to a nanoscale central region. By scanning this minimized fluorescent spot across the sample, STED microscopy generates super-resolved images with spatial resolution beyond the conventional diffraction limit.