Matrix Assisted Laser Desorption Ionization Laboratory (MALDI-ToF MS)

Objective

MALDI ToF/ToF (Matrix Assisted Laser Desorption Ionization Time of Flight/ Time of Flight) Ultraflextreme instrument and it is used for the determination of the molecular mass is an mass spectrometric of different chemical and biomolecules such as protein, peptide, polymers, organic & inorganic molecules etc.

This Intrument with special facility to determine denovo protein sequence, peptide mass fingerprint(PMF). This Instrument is coupled with a Nano Lc (Liquid Chromatography) for PMF, denovo protein sequence etc.

1. Accurate mass determination.
2. Peptide mass fingerprint.
3.Identification of microorganism using biotyper.
4.Tissue imaging using image prep.
5. Lipid identification using TLC MALDI.

Equipment & Details

MALDI ToF/ToF (Time of Flight/Time of Flight) Utraflextreme
    Name: MALDI ToF/ToF;  
    Model: Ultraflextreme  
    Manufacturer details :  BRUKER DALTONIK GmbH  Life Sciences  Fahrenheitstr. 4, D-28359 Bremen Germany , SALES CONTACT
    phone: +49 (421) 2205-0, SERVICE CONTACT
    phone: +49 (421) 2205 350
    Year of Instalation: 2014

Sample Details

Different Type of samples (Protein, peptide,polymer,chemical molecules ) are mixed with appropiate matrix (eg. SA, HCCA,DHB,etc) for crystallization before data acquisition.

Fig: True spectra of Maldi Tof/Tof  PMF

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Assigned Technician:

Utility and Working Principle